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JA Moshier E Malecka-Panas H Geng J Dosescu J Tureaud M Skunca AP Majumdar 《Canadian Metallurgical Quarterly》1995,55(22):5358-5365
Ornithine decarboxylase (ODC) has been shown to be oncogenic in transfected NIH/3T3 cells overexpressing the enzyme from a heterologous promoter. These cells, designated as NODC-2 cells, acquire proliferative properties associated with tumorigenic transformation such as loss of contact inhibition, decreased population doubling time, anchorage-independent growth, and tumor production in nude mice. At least one of these parameters, loss of contact inhibition, remains dependent on elevated ODC levels. We have used these cells to investigate the molecular mechanisms by which ODC overexpression drives cell transformation and to examine the involvement of other proto-oncogene products in this process. An interaction between ODC overexpression and the epidermal growth factor receptor (EGF-R) was suggested initially by the elevation of both basal (300%) and ligand-induced (457%) EGF-R tyrosine kinase activities in NODC-2 cells compared to similarly treated control NLK cells. Disruption of EGF-R mediated signal transduction in NODC-2 cells both by treatment with tyrphostin-25 or by transfection with a vector expressing a dominant negative EGF-R mutant resulted in reacquisition of contact-inhibited growth and suppression of anchorage-independent, clonogenic growth in soft agar. We conclude that ODC-induced transformation of NIH/3T3 cells is mediated, at least partly, by alterations in EGF-R signal transduction activity. 相似文献
24.
A nanocrystalline coating of K38G alloy was obtained by means of magnetron sputtering. The corrosion behavior of cast K38G alloy and its sputtered nanocrystalline coating with a solid NaCl deposit in dry air or wet oxygen at 600 and 700°C have been studied and compared. The results indicated that the mass gain of the coating is higher than that of the cast alloy at 600 and 700°C. Therefore, nanocrystallization reduced the corrosion resistance at these temperatures. Furthermore, the mass gains of the coating and the cast alloy with a NaCl deposit in wet oxygen were less than that with a NaCl deposit in dry air. The relevant corrosion mechanisms are discussed. 相似文献
25.
无线局域网中基于RC4的加密算法的分析与改进 总被引:2,自引:0,他引:2
分析RC4加密算法自身的缺陷和无线局域网WEP协议对RC4的误用,介绍了TKIP协议在安全性方面的改进。 相似文献
26.
对薄板成形应变场传统的测量方法进行了研究。指出了其不足和误差的来源,提出了数字图像分析法测量薄板成形中的应变场,对测量原理,新的测量方法对传统方法的改进,以及如何降低误差进行了介绍,指出数字图像分析法的前景,提出了改进意见。 相似文献
27.
新型可调剪切差平行分束偏光器设计 总被引:4,自引:2,他引:2
本文给出了一种新型可调的切差平行分束偏光器设计。它调节方便,可获得较大平行分束剪切差,并可一镜多用 相似文献
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The formation of a low Cr-volatility and electrically conductive oxide outer layer atop an inner chromia layer via thermal oxidation is highly desirable for preventing chromium evaporation from solid oxide fuel cell (SOFC) metallic interconnects at the SOFC operation temperatures. In this paper, a number of ferritic Fe–22Cr alloys with different levels of Mn and Ti as well as a Ni-based alloy Haynes 242 were cyclically oxidized in air at 800 °C for twenty 100-h cycles. No oxide scale spallation was observed during thermal cycling for any of these alloys. A mixed Mn2O3/TiO2 surface layer and/or a (Mn, Cr)3O4 spinel outer layer atop a Cr2O3 inner layer was formed for the Fe–22Cr series alloys, while an NiO outer layer with a Cr2O3 inner layer was developed for Haynes 242 after cyclic oxidation. For the Fe–22Cr series alloys, the effects of Mn and Ti contents as well as alloy purity on the oxidation resistance and scale area specific resistance were evaluated. The performance of the ferritic alloys was compared with that of Haynes 242. The mismatch in thermal expansion coefficient between the different layers in the oxide scale was identified as a potential concern for these otherwise promising alloys. 相似文献
29.
����ѡ������Ļ�ɫ���߷��� 总被引:2,自引:0,他引:2
本文根据灰色系统理论中的灰色决策原理,提出了一种合理选择钻机的新方法,并通过了实例验证。结果表明,这种方法切实可行。 相似文献
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